Ligated samples were run on a 2% agarose gel and DNA fragments of the desired size extracted using a Gel extraction kit (Qiagen).
The PCR products were resolved by electrophoresis on 15% polyacrylamide gels and DNAs of the appropriate size extracted using QIAquick Gel Extraction Kits (Qiagen).
Maximum lycopene (1.98 mg/100 g) was extracted when the solvent/meal ratio, number of extractions, temperature, particle size and extraction time were 30:1 v/w, 4, 50 °C, 0.15 mm and 8 min, respectively.
An automatic variable size part extraction framework iteratively refines the object's roughly aligned initial pose due to the extraction of coarsest parts, the ones occupying the largest area in image pixels.
An equation to estimate the costs of industrial size supercritical extraction systems was developed and used to estimate the cost of manufacturing (COM) of habanero pepper oleoresin on extraction cells ranging from 5 to 400 L.
If you want to learn how grind size affects extraction, here's your chance.
The optimum parameters, such as temperature, particle size, and extraction time were in turn 20 °C, 80 mesh, and 8 h.
Total phenolic content (TPC) of yellow, green and black tea leaves was from 0.575 g/kg to 6.629 g/kg, depending on particle size and extraction solvent.
