Cell counts and purity were measured using an electronic cell counter equipped with a cell sizing attachment (Coulter model Multisizer II with a 256C channelizer; Coulter Electronics, Hialeah, FL).
Ring size and attachment points were determined by methylation analysis as described [13].
Rafts are now defined to have diameters of just 10 200 nm (Keystone Symposium on Lipid Rafts and Cell Function; [19]), but published reports of confocal imaging studies using fluorescent raft components [69] report sizes (∼1 µm) similar to those found here and likely reflect amplification of apparent size by attachment of multiple fluorescent molecules and/or aggregation of smaller raft domains.
These cell index values are then directionally proportionate to cell number, size and attachment.
Of the three related factors, tumor location probably weighted more than GTV size and attachment degree to rigid structure.
In the present study, our analysis revealed there was a weak positive correlation between tumor motion and the SI tumor location, but the relationship of tumor motion and GTV size or attachment status was not linear.
Other factors including age, gender, tumor size, tumor attachment, and petrosal vein compression by the tumor did not have a significant impact on the incidence of postoperative venous complications (P > 0.1 for each comparison; data not shown).
The CI value is defined as relative change in measured electrical impedance to represent cell status, and is directly proportional to quantity, size, and attachment forces of the cell.
For coculture experiments, astrocytes (1.33 × 10 cells/cm) were seeded onto po/ln-coated trans-well inserts (GreinerBioOne, Monroe, NC, 0.4 μm pore size); after attachment (overnight) in astrocyte medium (ScienCell) the inserts were washed twice with stem cell medium and transferred to multiwell plates carrying GSCs.
Repeatabilities were based on the variance components derived from an analysis of variance (ANOVA), with equal sample sizes (courtship duration) and unequal sample sizes (spermatophore attachment) [ 40].
