In the specific situation of cell therapy for diabetes, it is not necessary to achieve functional integration with the host tissue since the cells are aimed to serve as glucose sensor and insulin producers, and their contact only via with the blood circulation should be sufficient.
However, measuring biophysical and biochemical parameters in the heterogeneous situation of cell migration is a difficult task.
A study by Zhao [ 23] examined differences between T7-based amplification protocols, including the Eberwine method and the template switch (TS) technique, where these methods were used to amplify RNA extracted from tumor samples rather than from the idealized situation of cell lines.
However, energy charge as calculated value from the absolute concentrations of AMP, ADP and ATP has been proven to be a valuable parameter for energetic situations of cells and tissue [ 24- 26].
To mimic this situation, images of cells treated with the highest concentration of TNFα and stained with NAO and DRAQ5 were used to train an SVM, as above, which was then applied to all of the other sets of images.
Autophagy plays a vital role in the liver and in hepatocytes both under basal conditions and in situations of cell stress [ 38].
This model reflects best the situation of MRD cells in vivo, which invade and remodel the surrounding tissue.
Considering the technically important situation of constant cell voltage, the activation energy for PEFC is decreasing more rapidly due to the temperature induced decrease of the reversible thermodynamic cell voltage.
These models are no mutually exclusive, and their relative contribution to the pool of cytosolic CRT may depend on the physiological situation of the cell.
