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Although we presented representative in-game decision-making situations, there might still be expertise-based differences between these film-based situations and in situ situations (Dicks, Button, & Davids, 2010).
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The density can be dry if the pore space is empty, or it can be saturated if the pores are filled with fluid (e.g., water), which is more typical of the subsurface (in situ) situation.
This potentially could provide a much more simple testing approach with the capacity for in situ situation where standard testing specimens are not attainable.
With regard to the "real in situ situation," no corresponding removal forces are present in the peri-implant region, which is protected from such forces by the adjacent peri-implant mucosa.
This discrepancy indicates that extrapolation of the in vitro data to the in situ situation should be done with caution.
These results can only be extrapolated to the in situ situation if COX is expressed in the intact growth plate.
Therefore, the extrapolation from in vitro data to the in situ situation and the interpretation regarding physiological processes must be done with caution.
Because in our culture system proliferative cells represented the majority of chondrocytes, the ubiquitous expression of EP1 receptor in vitro was in contrast to the in situ situation.
These data demonstrate that direct extrapolation of in vitro cell age responses to the in situ situation may not always be valid.
Paralleling the in situ situation, both COX-1 and COX-2 mRNA as well as COX-1 and COX-2 protein were expressed in cultured chondrocytes.
However, inadequate information is available on in situ expression of both COX-1 and COX-2 within the growth plate to correlate in vitro findings with the in situ situation.
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