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In a more sophisticated approach, probes can be synthesized in situ on the array surface using ink-jet or light-directed oligonucleotide synthesizers, thereby bypassing the need for a secondary linkage reaction [19], [20].
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For each gene, eleven pairs of oligonucleotide probes are synthesized in situ on the arrays.
For each gene, eleven pairs of oligonucleotide probes were synthesized in situ on the arrays.
However, in contrast to mRNA arrays, which have an exceedingly high density of probes that are in situ synthesized on the array; miRNA microarrays are often lower density spotted arrays [ 8].
The most optimal probes sets per sequence were selected for in situ synthesis on the marmoset array.
We conclude that the in situ patterns confirmed the array predictions for 27 of 29 genes.
Probes were synthesized in situ by photolithography on glass slides using a computer-generated randomized pattern on the array.
All probes are replicated once on the array for a total of over 385,000 probes synthesized in situ on glass slides [ 64].
glaberrima sequences printed on the array.
The density of features on the array varies between platforms, from 20 000 spots per slide for a typical spotted array, to several million for platforms such as NimbleGen and Affymetrix that use in situ synthesized oligos.
Ø : Isoform not represented on the array.
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