Notably, one DR sequence and two BsmBI sites were introduced after the J23119-SpeI promoter, and the two BsmBI sites facilitate further insertion of different guide sequences.
Thirteen unique silent restriction sites were introduced in the pol gene encoding HIV-1 RT, allowing easy introduction of mutations.
For easy cloning of any antibody gene without potential modification of the antibody sequence, restriction sites were introduced in the pelB sequence and following the termination codon.
The loxP sites were introduced either into the intronic region of early genes or between the two poly(A) signal sites of convergent transcriptional units.
The native cysteine residues of green fluorescent protein (GFP) at positions 48 and 70 were replaced by non-thiolic amino acids, and new cysteine sites were introduced at specific, surface positions.
De novo Zn II -binding sites were introduced directly at the surface of both fluorescent domains of a chimera of enhanced cyan and yellow fluorescent protein, connected by a flexible peptide linker.
Through PCR EcoRI and NheI sites were introduced and then ligated to the corresponding sites of p2159 and p2158.
Eco RI and Bam HI sites were introduced to generate MADS-deleted CsAP3 and CsPI, for cloning into pGADT7 and pGBKT7, respectively.
The appropriate restriction sites were introduced into the PCR product for subsequent cloning steps (Spe I and Kpn I at the 5′ end and Sac I and BamH I at the 3′ end).
Nde I and BamH I sites were introduced in forward and reverse primer respectively.
PmeI and NheI restriction sites were introduced in the primer as cloning sites for NR ligand binding domains.
