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Except for PMT1A BglII sites were integrated into the PMT ORFs using oligonucleotide based site directed mutagenesis (Stratagene Quick Change site directed mutagenesis kit).
Identified gene pairs and their conserved binding sites were integrated into the system.
The genomic positions of miRNA target sites were integrated into AthaMap, a database for predicted transcription factor binding sites in the A. thaliana genome [ 22].
The failure to detect recombinase activity mediated by the φ370, FC1, TG1, RV, FC1, φK38, MR11, A118 and BL3 integrases in HT1080 cells using deletion substrates integrated into the vertebrate genome posed the question as to whether these integrases were active in HT1080 cells or whether they were simply prevented from acting by the fact that the target sites were integrated into the genome.
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The concepts of thresholds for growth, axillary spread, and distant sites are integrated.
She shared with us a new statistic: 88% of Internet Retailer Top 200 retail sites are integrated with Facebook.
That is why EMRs of several sites are integrated by an entity named as electronic health record (EHR) (explained below), and the EHR is then shared by Care Delivery Organizations (it is also detailed below).
The display of single and paired CRISPR sites is integrated with pre-computed off-target scores and user-controlled filters.
Importantly, epidemiological studies at the ICEMR field sites are integrated with entomological studies, including the measurement of the entomological inoculation rate, human biting index, and insecticide resistance, as well as studies of parasite genetic diversity and antimalarial drug resistance.
Importantly, epidemiological studies at the ICEMR field sites are integrated with entomological studies, including measurement of the EIR, human biting index, and insecticide resistance, as well as studies of parasite genetic diversity and antimalarial drug resistance.
Briefly, a rad60 haploid 'base strain' was created as follows: the loxP site was integrated 300bp upstream of the rad60 coding sequence, and ura4+ and the loxM3 site were integrated immediately downstream of the rad60 coding sequence.
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