Sentence examples for sites were inserted from inspiring English sources

To provide convenient restriction enzyme sites for recombinant plasmid construct, NdeI/XhoI sites were inserted into the 5′- and 3′-ends of the mu-IFN-CSP gene.

Restriction endonuclease BamH1 and XbaI sites were inserted into pCMV-BGH3'.

Step 1: Single loxP sites were inserted flanking the targeted ∼42 kb genomic region by recombination of PCR products using a modification of the Lambda-Red recombination method [23].

A targeting vector containing a neomycin resistance gene flanked by lox P Cre-recombination sites were inserted into the intron downstream of the GASP open reading frame (ORF) (intron 5).

Cannulae for medial olfactory tubercle and medial nucleus accumbens shell sites were inserted at a 20° angle from the other hemisphere through the midline to minimize diffusion of drug solution to the shell or core, respectively (Fig. 1A).

Two TetO2 sites were inserted by PCR into each of the three reporter plasmids 10 bp downstream of the TNF-promoter TATA box to generate TNF-TetO2-DTA, TNF-TetO2-Tox176, and TNF-TetO2-EGFP.

To construct the reporter cassette insertion plasmid, a linker containing AscI, SpeI, BglII, NdeI and SphI sites was inserted between the XbaI and SacI sites of pBluescript II SK (Stratagene) to construct the pT1 vector.

While new sites are inserted, a flooding-based remeshing algorithm is called to optimize the location of all sites and achieve the remeshing results iteratively.

The assay is based on the induction of expression of a firefly luciferase reporter by a chimeric transcription factor in which the viral protease and cleavage sites are inserted between the GAL4 binding domain and the VP16 activation domain.

For positive selection, the Pgk-neo gene cassette flanked by loxP sites was inserted at the EcoR1 site in the 3' non-coding region of APP-exon 18.

The nucleotide sequence of eGFP flanked by NotI and XbaI restriction sites was inserted downstream of the rhodopsin and linker sequences.