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The enzymic properties including the substrate specificities at the P1 and P1′ sites were determined using a competitive hydrolysis method employing synthetic substrate mixtures.
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Potential crRNA target sites were determined using an algorithm provided by the CRISPRscan.org website34 and sites were chosen within the first 200 bp of the gene.
Annual average fractional available volumetric water content (θa), total annual water loss and total annual drainage for each treatment at each of the eight sites was determined using a daily water balance model, validated against two-monthly measurements of θa.
In MethLAB, the phenotype dependent differential methylation p-value at each CpG site is determined using a linear model [ 23].
The relative elevation of each coring site was determined using an automatic level, based on the water level of the Blackwater Lakes at 6 00 P.M. on 21 August 2011.
In general, the reference profiles of YSR for these sites were determined using series of laboratory consolidation tests on undisturbed samples at varied elevations, coupled with a good understanding of the engineering geology background of the formations.
Differences between sites were determined using ANOVA followed by Tukey Kramer post hoc tests to find groupings.
Locations of selected sampling sites were determined using the Garmin Vista CP GPS (Fig. 2).
The topographical factors of 9 shallow landslides and 10 potential sites were determined using the digital elevation data.
The exact locations of sites were determined using the mobile Global Positioning System (GPS) device according to theoretically calculated coordinates.
Low energy adsorption sites were determined using Adsorption Locator and Forcite modules of Materials Studio 6.0 (Accelrys Inc .. Subsequently, the structures were optimized with DMol3 code (Accelrys Inc).
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