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The summed Kullback-Leibler distance was used to quantify the difference between the predicted DNA binding sites of the reference and SNP forms of the zinc finger.
First, a relatively short read naturally has more chances to be aligned to multiple sites of the reference genome and thus to be filtered out as an "ambiguously mapped read".
A substantial proportion of reads were mapped to many sites of the reference sequences: 41% reads in mapping among ecotypes and up to 47% reads in mapping of ecotype onto WGS.
Despite the GPKB integrates all data publicly available on the FTP sites of the reference Entrez Gene and GOA databases, as well as of some relevant gene pathway databases (i.e. BioCyc, KEGG and Reactome), by using the transitive relationship approach we could detect and transfer numerous gene pathway and biological function (Gene Ontology) annotations not included in these databases.
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The sequence 5′ of the translation start site of the reference ORF was then deleted.
The binding site of the reference inhibitor is well defined by hydrophobic cavity.
The CWCS was then calculated as log2(1 − C n ), in which C n was the C i at the most proximal site of the reference 3′ UTR.
10 reads were mapped to the second nucleotide site of the reference transcript AT5G54320.1 and eight of these reads reported "A", therefore, the second site of this transcript was assigned as "A".
The projection shows the probability of observing a derived allele at a particular site in a test genome, relative to the derived allele frequency at that site of the reference population.
We corrected the SNP sites of the Nipponbare reference genome (http://rice.plantbiology.msu.edu, The MSU Rice Database release 7.0) according to the sequences of Zhenshan 97 and Minghui 63 to reconstruct 'SNPs-replaced reference genomes' for the two parents.
Comparisons of the binding site to the reference structure, here FLT3, can be displayed, along with root-mean-square deviation values, in this case indicating that the binding sites of these protein kinases are highly conserved.
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