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The nanopores and acid sites of modified zeolites were extensively investigated by N2 adsorption, XRD and in situ IR methods.
Chromatin Immunoprecipitation coupled with high throughput sequencing (ChIP-seq) allows the study of binding sites for TFs or sites of modified histones to be identified in a single experiment on a genome-wide scale.
The amplified open reading frame (ORF) of hnRNP A1 was subcloned into Bam HI and Hind III sites of modified pGEX-6p-1 vector to create recombinant E. coli expression vectors for gluthathione S-transferase (GST) full down assay.
The dependence of mCpG binding specificity on either salt or the presence of competitor shows that suppression of nonspecific binding underlies the ability of the MBD to discriminate sites of modified nucleotides at physiological salt concentrations.
The least conserved region (mRNA+685 to +1476, amino acid 229 492) of FoxO6 was inserted into the BamHI/EcoRI sites of modified pET-32a vector, in which the 3′-His-tag was made in-frame by blunting the NotI/XhoI sites, to generate FoxO6 (229–492) protein with His-tag on both ends.
To assess the bindings of epigenetic markers to background genes (all the 25363 human genes from UCSC hg18), we used each human gene region plus its 100 bp bases before transcription start site to map to the binding sites of modified histones and DNase.
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(i) Flexibility facilitates the deposition of PTMs by enabling transient but specific interaction with catalytic sites of modifying enzymes.
Based on the SNP sites, four pairs of modified specific primers were designed respectively for authentication of Hippocampus kuda, Hippocampus japonicus, Hippocampus trimaculatus and Hippocampus kelloggi using allele-specific PCR.
Similar to acetylation and phosphorylation, site-specific detection of modified residues in target proteins would be the most rigorous method to unequivocally identify modified proteins, but this is still challenging.
During this period, new genetic and genomic resources and technologies have been developed that allow tissue-specific knockdown of gene expression, gene replacement and site-specific integration of modified and/or exogenous genes in the Drosophila genome.
Subsequently, to block the non-specific sites of the modified electrode, GOx was employed instead of the usual sealers.
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