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The number of transgenes that have been inserted and their sites of insertion are uncontrolled.
Firstly we report the elements inserted in the same genome context in different strains, indicating preferred sites of insertion; that, the ones related to common ancestral events.
Sites of insertion included the tibia and the humerus.
We proposed to analyze the complications related to the maintenance of supraclavicular catheter versus the others sites of insertion.
As compared to mutations generated by classical methods of chemical treatment and ultraviolet irradiation, transposon insertions are easy to identify: the transposon-encoded sequence serves as a marker or tag through which sites of insertion may be rapidly identified by polymerase chain reaction (PCR) amplification or plasmid rescue.
There are seven sites of insertion shared by prophage elements from two different strains.
Similar(26)
We focused on identification of simple sequence repeats (SSRs) and sites of insertions of transposable elements (ISBP - Insertion Site Based Polymorphism) [ 43].
However in most real life cases, we do not have multiple experiments and do not know the sites of insertions or non-insertion sites in advance.
The presence of target-site duplications at the sites of insertions of some TCAST transposon-like elements also indicates transposition as a mode of spreading of TCAST elements.
The tibia was the first site of insertion, and a second IO was inserted in the humerus if clinically indicated for the same patient.
The sensor was inserted 24 h prior to each main trial, with the site of insertion replicated between trials.
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