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Tamoxifen was injected subcutaneously on the inside of either thigh with the sites of injection being rotated.
The only difference is that we did not target TrPs as the sites of injection.
It can form nodules subcutaneously, particularly at the common sites of injection, namely the inframammary crease or axillary region.
This could be local or generalized adverse events such as indurations; damage to nerves and blood vessels; abscess formation around the sites of injection etc.
The most effective dose, volume of dilution, number and sites of injection required for effective treatment are not well known [3].
So, we targeted sites of myofascial pain, which are thought to contain TrPs [22] but did not make any effort to target TrPs themselves as sites of injection.
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The most common side effects were indurations and erythema at the sites of injections.
During this period, the injector was allowed to modify the dosage and sites of injections.
The sites of injections were additionally dyed with trypan blue to improve further localization.
This difference, most likely, is due to the subcutaneous versus intraductal sites of injections.
The injected cells migrated from the site of injection to the epithelium (Supplementary Figure S4B).
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