Fascin-1 was widely expressed throughout the embryo and the developing nervous system and mesenchymal tissues represented major sites of expression.
Further sites of expression are the boundary of the early neural plate and surface ectoderm, the roof of mesencephalon, pretectum and dorsal thalamus, the differentiating heart, and the otic vesicle.
Specific primers containing Xho I and BamH I restriction sites respectively, were designed based on the obtained ORF sequence of LvCHH gene and the cloning sites of expression vector pET-32a.
According to the sequence of the bile salt hydrolase (BSH) gene of Bifidobacterium and the restriction enzyme cutting sites of expression vector pNZ8148, primers were designed and the bile salt hydrolase (BSH) gene was gotten from Bacillus bifidus ATCC 29521 by PCR.
Other sites of expression include the anterior CNS and branchial arches.
Sites of expression in the mouse embryo were consistent with previously described sites of Sox2 mRNA.
For example, the posterior mesoderm, the paraxial mesoderm and the tailbud are common sites of expression.
While the intensity of the reporter gene expression seen in the transgenic fetuses varied, the sites of expression did not.
It might be the case that these transcripts undergo dynamic translation at sites of expression in foot processes in response to extracellular cues and signaling pathways.
The PCR-amplified DNA fragment was cloned into complementary sites of expression vector pET14b (Novagen, Germany) to produce the recombinant plasmid pET14b xyla5-14.
Other sites of expression are human keratinocytes, where meprin α and β are found in the stratum basale and in the stratum granulosum, respectively [11].
