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We then mapped the motif across the entire X chromosome and calculated the average distances to transcription start sites of expressed genes strongly bound and weakly bound by the MSL-complex.
Genomic regions around transcription start sites of expressed sLAD genes display reduced association with the NL; additionally, expressed sLAD genes possess lower levels of active histone modifications than expressed non-sLAD genes.
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We have shown that GIS-PET is able to precisely locate the transcription start sites (TSSs) and the polyadenylation sites (PASs) of expressed genes.
For many transcription factors, DNA polymerase II, and some modified histones such as tri-methylated H3K4, the majority of binding sites are located near the transcription start sites (TSS) of expressed genes.
For example, we have detected substantially lower NL association around the transcription start sites (TSSs) of expressed sLAD genes.
Moreover H4K16ac and Mof were reported to be present at the transcription start sites (TSSs) of expressed genes in mESCs (Li et al., 2012; Taylor et al., 2013).
However the divergence at synonymous sites of lowly expressed genes was not significantly different to that of the highly expressed genes (0.464 vs 0.458 P = 0.58).
To do that we used as a proxy the number of binding sites for expressed miRNA present in transcription factors 3′ UTRs.
Class I sites cluster around the transcriptional start sites of highly expressed genes with no chondrocyte-specific signature.
Here, we describe the installation of methyl-lysine analogues (MLAs) as a simple and scalable method to introduce mono-, di-, or trimethylation at specific sites of recombinantly expressed histones.
Recently, a histone signature comprising 17 modifications has been shown to mark the transcriptional start sites of highly expressed genes in T lymphocytes [38].
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