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delivery, which are the sites of administration for the Biothrax™ and rPA102 vaccines [4], [36].
Further studies are needed to evaluate the tissue specificity and pharmacokinetic characteristics of POH to determine its most appropriate clinical uses, as well as identify the most appropriate sites of administration to avoid unwanted accumulation or rapid excretion, based on the target tissue.
The anatomical sites of administration were not available.
Using the linear mixed model, we calculated comparable BVs in the two sites of administration (estimate = 0.43 l, SE = 0.27, p = 0.126), as well in the two different doses (estimate = 0.02 l, SE = 0.28, p = 0.953).
The flank vaccination site was only tested on the 8.0 logroupsID50/mL groups, as we expected them to have the highest immune response; however there was no significant difference between the two sites of administration.
It is likely that more efficient administration of the PfSPZ contributed to the delay by increasing the number of sites of administration from two to four and decreasing the volume of the injections from 50 to 10 μL, as we have seen in mice.
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In general, these processes are: (1) administration of the drug, (2) absorption from the site of administration into the bloodstream, (3) distribution to other parts of the body, including the target site, (4) metabolic alteration of the drug, and (5) excretion of the drug or its metabolites.
The site of administration is another important factor to be considered in the experimental design.
Simultaneously clearance of gels from the site of administration was monitored non-invasively using MRI.
Local tolerance studies provide information on tissue reactions at the site of administration.
The challenge of a gene carrier is to deliver exogenous DNA from the site of administration into the nucleus of the appropriate target cell.
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