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In all structures, substrates and effectors were found ordered at their respective binding sites (loop 2 electron density shown in Figure 2).
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Lac repressor (LacI) binds two operator DNA sites, looping the intervening DNA.
Removal of the insertion at the active site loop caused production of an inactive enzyme.
Intrinsic and extrinsic fluorescence analysis indicated change around active site loop region having single tryptophan residue.
However, the reactive site loop involved in SCCA2(S) is an important factor in restraining serine proteinases.
We propose a helix-to-loop conformational switch in the helices flanking the inner active-site loop that regulates accessibility of the active site.
In particular, these studies have pointed toward highly conserved structural elements, like the motif-D active-site loop, that can be modified to generate polymerases with desired properties.
Our computational results suggest that mutant residues Ile35 and Lys216, and active site loop L1 residues 65 73 in NDM-1 play crucial roles in substrate recognition and binding.
Differences at the active site loop of the host and parasite LDH's makes this enzyme an attractive target for drug inhibitors.
Most of the sequences showed variation (2 to 20%) in the deduced AA sequences which were pronounced close to the reactive site loop.
A peptide was designed from reactive site loop structure of α1 Antitrypsin Portland known as α1 PDX as a novel mini-PDX inhibitor of furin.
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