Exact(1)
The data matrix was made with 63 taxa and 437 amino acid sites (available upon request to SM).
Similar(59)
Additional data sources included reference lists of relevant reports that were searched manually, as well as key experts, organizations, and web sites (list available upon request).
Rainfall is heaviest from May to September and a dry season extends from November to February (climatic records taken at the site are available upon request).
The cDNA of CG8316 (Berkeley Drosophila Genome Project, Drosophila Gene Collection #LD15362) was amplified with primers containing NdeI and BamHI sites (primer sequence available upon request) and subcloned into pET19b at NdeI and BamHI sites within the multi-cloning region.
The SGF73 promoters and coding sequences were amplified from yeast genomic DNA using primers containing a 5' SmaI site and a 3' SmaI site (primer sequences available upon request).
Site classifications are available upon request.
The BfaI site originally located in the PCR fragment from genomic DNA was deleted by site directed mutagenesis (details available upon request).
Specific primers for each binding site were designed (primer information is available upon request from the corresponding author).
For cloning purposes, primers (sequences available upon request) contained restriction sites.
DNA was extracted [ 25] and the digest was evaluated by qPCR with primers amplifying around the transcription start site of the mouse Rpl19 gene (primers available upon request) and human RPL19 gene (for the mouse primers sequences see Additional file 9: Table S4), giving a product of approximately 100 bp.
Site-directed mutagenesis of the HA cleavage site region was performed by the Quikchange™ protocol (primer sequences available upon request).
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