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The protocol was approved by the institutional review boards of the participating sites, a protocol review committee, and a data safety monitoring board.
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As the IADPSG criteria are not used to diagnose GDM at all sites, each site has a protocol on how to link women with appropriate services in their locality.
The percentage of EIP sites having a protocol to guarantee chain of custody for food environmental specimens was nearly the same as for non-EIP sites (38% vs. 36%).
The γ vector was reconstructed to include PCR-ready cloning sites following a protocol modified from Holzberg et al. [ 10].
To ensure uniformity of data collection across the sites, a standard protocol was developed based on the protocol of an existing global collaboration on nutrients in processed foods [ 9] – Food Monitoring Group.
Constant velocity SMD simulations were used to explore the extracellular translocation pathway and the S2 site following a protocol described previously for a similar study of LeuT [29].
Labeled cDNA samples were hybridized to a custom-designed DNA oligo microarray (Agilent) (each gene is covered by 9 11 probes located −300 to +200 relative to the translational start site) using a protocol previously described [ 47, 48].
If needed, an additional 40 U of onabotulinumtoxinA or placebo could be administered among three muscle groups (occipitalis, temporalis, or trapezius; a total of eight sites) using a protocol-defined, follow-the-pain strategy.
A complete list of differentially expressed nTARs can be found in Additional file 5. To further corroborate polyadenylation sites, an additional protocol was used based on 3'-anchored pyrosequencing on a 454/GS-FLX [ 31].
After an initial review of current physiotherapy practice at the lead site, a draft protocol structured to reflect successive phases of rehabilitation was drawn up by four specialist physiotherapists from different centres.
To demonstrate the capacity of the device for delivering tactile stimulation at four independent sites, a finger agnosia protocol, in the presence and absence of conditioning stimuli, was conducted on seventeen healthy control subjects.
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