Exact(3)
It has been suggested that the branch site test of Zhang et al.[ 42] is not statistically robust when the number of substitutions in the MSAs is small [ 78].
Table S3 (Additional File 5) summarises the likelihood ratio test results for the branch test (Model H versus Model H null) and for the strict branch positive site test of positive selection (Model A versus Model A null).
The PAML branch site test of positive selection (Yang and Nielsen 2002; Yang et al. 2005; Zhang et al. 2005) detects positive selection at a subset of sites in a specific lineage.
Similar(57)
Open image in new window Fig. 18 Left: Construction of double layered, photocatalytic concrete road at industrial zone "Den Hoek 3" in March 2011; right: "On site" testing of photocatalytic efficiency.
Test 2, the branch-site test of positive selection, compares model A [ 23, 24] with model A with ω2 = 1 fixed (null model).
We performed the Test 2 or "branch-site test of positive selection" [ 51, 52] (see Methods) contrasting the model A against itself with ω2 fixed to 1 for each PD branch as defined in Figure 4 (FishBrain, FishTj, or MERPs).
More recently, Fletcher and Yang [40] showed that alignment errors can lead to a high number of false positives for the branch-site test of positive selection.
We used the likelihood-ratio test 2 (i.e., the branch-site test of positive selection) constructed from this branch-site model [47].
However, under certain circumstances the branch-specific test of selection can lack power and so we also used the branch-site test of selection [26], [30] implementing the Bayes empirical Bayes (BEB [26]) method to identify sites under selection.
Using branch-site model A with the Glires branch (see Figure S7B for labeling) as foreground lineage in the branch-site test of positive selection (null model ω2 = 1 fixed) LRT provided marginally significant support (P = 0.052) for 2 positively selected sites with Bayes empirical Bayes (BEB) probability above 0.7 (Table 2).
Our test used a site-by-site test of multinomial homogeneity as described by Wolpert [ 19].
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