Sentence examples for site specific restriction from inspiring English sources

Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) using site specific restriction enzymes were implemented for polymorphism study of TNF alpha promoter.

Genotyping for FCGR3A-158V/F FCGR3A-158V/F1H/R wandperformed FCGR2A-131H/R FCGR2A-131H/R polymorphic site, followas by site sperformedestriction digestion using the genome of 187 Japanese patients with rheumatoid arthritis (including 23 who were anti-GPI antibydy PCRitive) amplificationese healthy individuals (including nine whofwere antheGpolymorphic positeve).

The -174G/C IL-6 promoter SNP was analyzed by PCR amplification and digestion with a site-specific restriction enzyme using previously reported methods [ 20].

The 869C/T SNP was analyzed by PCR amplification and digestion with a site-specific restriction enzyme in accordance with previously reported methods [ 13].

The in vitro cloning of DNA molecules traditionally uses PCR or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join these inserts to vectors with the corresponding ends [ 23].

Sites b5, b9, b12, b13, H110, and H205 are largely invariant among network members due to site-specific restrictions in E-box DNA binding and dimerization stability.

In contrast, models acknowledging site-specific restrictions of the amino-acid alphabet, as does CAT, can in principle correctly estimate this probability.

In the second step, specific restriction sites were incorporated for cloning into the pHAL30 phagemid vector.

In the second step specific restriction sites were added to the 5' end of the DNA fragments.

In both optical and restriction mapping, the landmarks correspond to the recognition sites for specific restriction enzymes.

Restriction site selection requires a specific restriction site within the region of detection: upon disruption, a gene or its fragment may lose the recognition site for a restriction enzyme, leading to a change in the restriction pattern.