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Degenerated primers based on the cleavage site sequence of the F0 gene were designed to detect specific sequences characteristic of virulent and avirulent strains of NDV.
We therefore modified the BC strain by changing the multibasic cleavage site sequence of the F protein to the dibasic sequence of avirulent strain LaSota.
We modified the cleavage site sequence of the NS2B NS3 junction region and then developed expression and purification procedures to prepare a covalently linked, single-chain, NS2B NS3pro K48A mutant construct.
The analysis of the F1/F2 protein cleavage site sequence of the four MG isolates showed that they share an original cleavage site motif formed by five arginines (R) 112R-R-R-R-R116 at the C terminus of F2 exhibiting an atypical Q/R substitution at position 114 followed by a phenylalanine (↓F117) at position 117 of the F1 amino terminus (Table 2).
The cleavage site sequence of the clade 2.3.2.1 viruses from Indonesia (PQREdelRRRKR↓G) differed from recent clade 2.1.3.2 viruses (PQRE SRR KKR↓G) by a Ser deletion at position 325 and a K328R substitution.
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The target site sequences of the cis-regulatory elements of these genes constitute the physical basis of the network architecture.
During the last years it has become increasingly clear that splice site recognition in mammals is not only dependent on the conserved 5'- and 3'-splice site sequences of the introns.
Interestingly, as previously reported for prmt5 mutants [ 25], we found that the donor splice site sequences of the splicing events affected only in prmt5 mutants, or simultaneously altered in prmt5 and prmt4a b mutants, displayed an under-representation of the consensus A and G nucleotides present in the −2 and −1 positions of the consensus donor splice site.
Mutation of the Cys Phe Lys Cys inner active site sequence of Ero1α to the Ero1β sequence (Cys Asp Lys Cys) substantially increases the oxidase activity of Ero1α [ 7].
The cleavage site sequence of APMV-5 conforms to the favored sequence motif for cleavage by the intracellular protease furin, R-X-K/R-R↓F [32].
Therefore there appears to be a correlation between ligand-binding site sequence of FYVE domains and the domain architecture of these proteins.
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