Exact(3)
Briefly, two allele-specific probes were designed for each CpG site on the array: one for the methylated sequence and one for the unmethylated sequence.
The Infinium HM450 BeadChip assay detects the proportion of molecules methylated at each CpG site on the array.
At each CpG site on the array, methylation status was determined based on intensity measures of two probes corresponding to unmethylated (U) or methylated (M) CpGs.
Similar(57)
A Wilcoxon Rank Sum (Wilcoxon RS) test was used to test the significance of the difference between ultrastable sites on the array and non-ultrastable sites on the array.
We then linked the UCSC Genome Browser annotation (version hg19 of the human reference genome, http://genome.ucsc.edu) to each of the CpG sites on the array [ 47].
All odds ratios were corrected for background enrichment, which is required because not all CpG sites on the array can become a tDMR as a result of the varying density of the chips.
We further analyzed the repetitive sequence composition in these 31,205 repeat junction site sequences on the array using the RJPrimer pipeline [ 8].
Interestingly, TDRD7TD is the first tudor domain described to date that preferentially binds to H3K9me2/3 versus the numerous other methyl-lysine sites on the arrays.
The strongest effects of genetic regulation act locally (cis), within regions that are in linkage disequilibrium with the CpG sites assayed on the array.
Yet, only 8.3% (3633/43778) of the CUX1 consensus sites present on the array were bound in vivo.
These genes had 3669 methylation sites measured on the array within and near the gene as provided by Illumina (Additional file 1: Table S2).
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