Sentence examples for site of the vector from inspiring English sources

To produce the transgenic rice line gNAS1 + gNAAT (Fig. 2b), a 7.6-kb barley HvNAS1 genome fragment [8] was excised using HindIII and inserted into the HindIII site of the vector pBIGRZ [1].

This fragment was digested by BamHI and ligated into the BamHI site of the vector pUC19UPDS.

The primers contain restriction sites for in-frame ligation into the multi-cloning site of the vector.

The concatemers around 500 bp in length were cloned directly into the MCS multi cloning site) of the vector pUC19.

For enhancer-blocking assays, PCR products (Table S5) spanning CTCF binding sites were cloned into the AscI site of the vector pNI [71], [72].

Next, R-CFP cDNA was released with NheI and XbaI (2044 bp) and ligated into the opposite cloning site of the vector.

This was cloned into the corresponding sites of the vector pGEX-6P-1 (GE Healthcare) in E. coli DH5α.

The PCR fragment was then cloned into the SphI-SalI sites of the vector pVH14.05.

This product was cloned by restriction and ligation into the HindIII and NotI sites of the vector pKLAC1 (NEB).

This product was cloned by restriction and ligation into the HindIII and NotI sites of the vector pKLAC1.

This product was cloned by restriction and ligation into the NdeI and BamHI sites of the vector pDEST15 (Invitrogen).