To further dissect the possible ubiquitination sites of RIG-I that are critically involved in antiviral signaling, we individually replaced the nineteen lysine residues in the RIG-I CARD domain with arginine.
Mutational studies have underscored the key role of conserved amino acids within the ATPase active-site of RIG-I.
The ocean is about 4,400 deep at the site of the rig.
Accessible to all online, the Environmental Response Management Application mapped the site of the rig in the Gulf, ocean currents, ship positions and the movement of oil.
One example is what is probably the Gulf of Mexico's longest-running commercial oil spill, at the site of a rig destroyed by an underwater mudslide during Hurricane Ivan in 2004.
The massive gush of oil spilling from the site of the rig that exploded last week exceeds the worst-case scenario predicted by oil giant BP when it filed its exploration plan with the government.
This latter finding, however, is consistent with the recent report that pppssRNA and short OHdsRNA bind to overlapping sites of the RIG-I RD, and that short dsRNAs will activate IFNβ via RIG-I upon transfection if they contain 5' mono-phosphates that apparently stabilize the dsRNAs intracellularly [25].
To test potential interaction site of EGCG in RIG-I, we tested RIG-I deletion mutants that can activate signal transduction in the absence of exogenous ligands.
I have to admit, I was surprised and pleased that it took us an hour of flying in our float plane over Breton Sound and Barataria Bay and across the marshes, bayous, barrier islands and open water that lie about 70 miles from the site of the Deepwater Horizon rig before we spotted any significant ribbon of oil.
While the Macondo Prospect (the geological site of the Deepwater Horizon rig) gushes oil at a flow rate of 15,000 psi, Smith says historical data put expected flow rates in the Alaska OCS at a much lower 6,000 psi.
[The Santa Cruz Sentinel] Underwater inspections at the site of BP's 2010 Deepwater Horizon rig disaster in the Gulf of Mexico have failed to identify the source of a persistent sheen on the surface, officials say.
