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According to their site of collection, the collected biological samples were pooled in three groups: (i) patients having pus and serositises, (ii) patient with urogenital infections (urine, vaginal, and urethral), and (iii) mucous membrane (skin and nostril) of healthy persons.
The study involved the use of data obtained from a quality assurance program based on anonymous human biological material collected in accordance with legislation at the site of collection; as a result, no ethics committee approvals were needed.
Most of the geologic samples collected at Desert RATS 2010 were well documented at the site of collection, and upon delivery to the Johnson Space Center the samples were given a preliminary examination.
Following physiological experimentation in situ and in the laboratory, all specimens were returned alive to their site of collection.
We describe technical limitations of hair analyses and variation in cortisol concentrations as a function of asynchronous hair growth, anatomical site of collection, and the amount and numbers of hair/s used for cortisol extraction.
The extreme sample diversity, likely caused by the widely varied parent material, land use at the site of collection (e.g., grazing, recreation, agriculture, etc)., and climate resulted in poor calibrations even for Ctotal, Corganic and Ccarbonate.
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This group of samples was collected at sunset and the soil pH for the sites of collection was determined as previously described [ 10].
After giving birth, we returned all the adult females and excess larvae to their sites of collection.
Authors frequently describe the same sites of breeding of A. dumetorum as the sites of collection of our confirmed A. orinus specimens, such as Shakhdara river valley, Barvoz forest and East of Kulyab.
Figure 1 shows the geographic distribution of Aotus species and sites of collection.
However, genetic differentiation between populations was independent of geographical distances between the sites of collection, as revealed by the Mantel test (r = 0.051, P = 0.468; Figure 2B).
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