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The major goal of this work is to elucidate the nature and extent of active site occlusion by neighboring subunits.
To monitor active site occlusion due to a complementary subunit, it is important to identify which of the gorge residues are affected upon tetramer formation.
The accessibility of an active site may be divided into three states based on the probability distribution of the active site occlusion (Fig. 7 A ).
Once the dominant configurations, i.e., the most populated "low energy" states, are identified, it will be possible to compute the probability of active site occlusion due to a complementary subunit and to correlate these structural features to function by, for example, calculating the reaction rates based on structures representing various conformational states.
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The results also lay the foundation for further study on the role of tetramerization in the catalytic efficiency of AChE, which may include rate calculations on several structures representing different active site occlusions.
(H ) The assembly-site occlusion model.
We will refer to this as an assembly-site-occlusion model (ASO).
In the acute setting, CT or magnetic resonance (MR) angiography can determine the site of occlusion, early recanalization and the presence of abnormalities in the proximal arterial tree such as stenosis, occlusion or dissection, pertaining to the cause of the stroke [ 24].
Local samples at the site of occlusion were immediately obtained using aspiration catheterization (TVAC; Nipro) after crossing the occlusive lesion.
For determining the site of occlusion, SWI exhibits better sensitivity and specificity than T2 gradient echo (Fig. 2) or 3D TOF imaging [17, 18, 19].
In previous studies, the approximate frequency of the increased DWI signal within the venous thrombus has been reported as 41%[32]], thus not truly providing significant added value since all cases showed T1WI or FLAIR signal changes at the site of occlusion.
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