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Yet, among all datasets, GATK had the highest medium site heterozygosity.
Median site heterozygosity was always higher for the South Kinabatangan population than for the West Alas population.
(i) Kernel-density distributions for site heterozygosity and (ii) minor allele frequency were not identical among the SNP datasets (Permutation test of equality, p < <0.001, Figure 5).
Kernel density plots of the minor allele frequency and site heterozygosity distributions were drawn in R with the 'sm' package [ 73].
Nucleotide diversity was assessed using two indexes: θW [ 31], an estimate of the expected per site heterozygosity, and π [ 32] the average number of pairwise sequence nucleotide differences.
This difference can be at least partly accounted for by the ascertainment bias and high average per site heterozygosity of the classical, early RFLP as well as Alu markers [ Bowcock et al., 1991; Jorde et al., 2000].
Similar(51)
Thus, the CLC dataset contained the lowest overall site heterozygosities among all datasets.
Furthermore, the slightly higher site heterozygosities in the South Kinabatangan population are also in agreement with previous studies using conventional genetic markers [ 52, 63- 65].
Thus, despite the two species having similar synonymous site heterozygosities, D. simulans clearly harbors more polymorphism, suggesting a larger N e for the X chromosome.
The normalized number of variant sites, heterozygosity, and MAFs were significantly different by functional SNP category in TERT and CLPTM1L (P values <0.01; Table 1).
The significantly reduced normalized number of variant sites, heterozygosity, and MAFs, and low differentiation among ancestral groups for the coding sites, particularly for non-synonymous sites, compared with non-coding and silent changes suggests purifying selection in TERT and CLPTM1.
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