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The spleen is a key site for removal of parasitized red blood cells, generation of immunity and production of new red blood cells during malaria.
The protease recognition site for removal of the N-terminal polyhistidine tag is shown in italics.
All PCR primers encoded a TEV cleavage site for removal of the His6 tag after protein purification (Table 1).
The spleen is a key site for removal of parasitized red blood cells, generation of immunity and production of new red blood cells during malaria [ 29].
The main site for removal of the chemicals before entering the environment is in WWTPs (Fig 3) by sorption on sewage sludge and loss via biodegradation.
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"Abuse of such processes is a well-documented phenomenon – one academic study based on Google's published information about copyright-based removals estimated that more than 50% of removal requests originated with competitors targeting each others' sites for removal from search results," said Fleischer.
Moreover, most of the cleavage sites for removal of the import sequences had not been determined experimentally.
6xHis-MBP-Chip205-436, 6xHis-MBP-LDB156-285 and 6xHis-Lip-SSDP1-92 were co-expressed with a bi-cistronic expression vector (including N-terminal Tobacco Etch virus (TEV) protease sites for removal of tags) in E. coli BL21-CodonPlus DE3 -RIL cells (Stratagene, La Jolla, California, United States) and purified BL21-CodonPlus DE3 -RILe excellson chromatography, aStratagened (FiedLa Jolla, California, United States
Expression constructs with affinity tags often include an engineered protease site for tag removal.
In this experiment, we designed a new construct containing a tobacco etch virus protease (TEV) cleavage site for proteolytic removal of the 6xHis-tag after purification.
GWI were found to be the dominant mechanism and site for QD removal compared with solid water interfaces (SWI) and pore straining.
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