Sentence examples similar to site directed screening from inspiring English sources

In order to investigate this, a number of site directed mutants were isolated in AapQ and screened for transport specificity by expression from a plasmid in the aap bra null mutant RU1722 (data not shown).

Four Lys209 neighboring sites were then selected and site directed saturation mutagenesis libraries were constructed for high through put thermostability screening.

For our BCR-ABL screen, we recreated a majority of the mutations using site directed mutagenesis of the native target kinase, and introduced the mutants into fresh cells.

The Countrywide Web site directed her to a government site, makinghomeaffordable.gov, she said.

Myc3-RHO1Q68H was created by site directed mutagenesis.

Site directed mutagenesis was performed by the Quik-Change Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA).

For the site directed mutagenesis we used the Quickchange Site Directed Mutagenesis Kit (NO. 200518, Stratagene).

This was done by site directed mutagenesis by using the QuickChange Site-Directed Mutagenesis kit (Stratagene).

In addition to a classical method of screening for a new microorganism harbouring an interesting protease from various environments [ 2], different molecular biology techniques, such as directed evolution [ 18, 19], site directed mutagenesis [ 20] and metagenomic analysis [ 21], have been used to engineer numerous proteases with improved or novel properties.

Here, we performed a site-directed screening technique, tethering-fragment based drug discovery, against wild-type and mutant forms of NDH-2 containing engineered active-site cysteines.

Conception and design: Carr AM, Murray JM, Fontebasso Y. Experimental execution: Fontebasso Y (standard genetic assays and setup of high-throughput genetic screens), Etheridge TJ (site-directed mutagenesis and generation of mutant strains), Oliver AW (generation of sequence-threaded homology models and design of mutants).