Sentence examples similar to site directed fluorescent from inspiring English sources
This step is followed by specific 16S rRNA directed fluorescent in situ hybridization.
Myc3-RHO1Q68H was created by site directed mutagenesis.
Site directed mutagenesis was performed by the Quik-Change Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA).
For the site directed mutagenesis we used the Quickchange Site Directed Mutagenesis Kit (NO. 200518, Stratagene).
This was done by site directed mutagenesis by using the QuickChange Site-Directed Mutagenesis kit (Stratagene).
We explored the molecular interface between CD81 and claudin-1 using a combination of bioinformatic sequence-based modelling, site-directed mutagenesis and Fluorescent Resonance Energy Transfer FRETT) imaging methodologies.
We investigated the molecular interface between CD81 and claudin-1 using a combination of bioinformatic sequence-based modelling, site-directed mutagenesis and Fluorescent Resonance Energy Transfer FRETT).
Site-directed fluorescent labelling is a powerful approach to provide insights into the processes occurring in ABC proteins upon substrate binding and transport, as well as nucleotide binding and hydrolysis.
The I130T GFP (green fluorescent protein) cDNA construct was generated using the QuikChange® site-directed mutagenesis kit (Stratagene) using the Cx43 GFP construct as a template and the primers 5′-CACTTGAAGCAGACTGAGATAAAGAAG-3′ (forward) and 5′-CTTCTTTATCTCAGTCTGCTTCAAGTG-3′ (reverse).
A combination of PCR-based manipulations, restriction digests, and site-directed mutagenesis procedures was used to mobilize the coding sequence and produces in-frame fusions with the affinity tags (GST, His X6 or FLAG epitope) or mCherry fluorescent tag, and to create the deletions and the point mutations indicated in the text.
NP swabs were tested for the presence of influenza by direct fluorescent antigen (DFA) detection or enzyme immunoassay (EIA) on-site in 5 of 6 hospitals, and by polymerase chain reaction (PCR) and viral culture at the Ontario Public Health Laboratory for all specimens.
