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"We make them fresh weekly with fruits and vegetables, essential oils and cleansing clays, and deliver them to the shops where they sit on ice, like a fresh buffet," Ms. Vega said.
His interrogators reportedly beat him so badly that most of his ribs were broken; they extinguished cigarettes on his genitals; they made him sit on ice cubes; they forced water down his throat so that he nearly drowned.
"Someone who can sit on ice cream and tell what flavor it is".
Samples were allowed to sit on ice (plasma) or at room temperature (serum) for 15 minutes, centrifuged at 4000 RPM for 15 minutes, aliquoted into 600 µL aliquots and frozen down at −80°C for later analysis.
To test how robust ExCyto PCR was if the reaction mix had to wait before use, we added 2 ul of ExCyto PCR cells to the reaction mixes and let them sit on ice for 30, 60, and 120 minutes (Figure 5).
Interestingly, pSTAT-5 expression was detected at much lower frequencies if cells from the thymus or from the periphery were allowed to sit on ice for an hour before fixation and staining (fig. 3A), showing that the results gathered on freshly isolated cells likely reflected a very recent signal received in vivo.
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That is where hope sits, on ice, getting freezer burn.
We sat on ice benches, once again insulated with deer pelts, where we studied our hands through the one-foot-thick lens of an ice-block table.
Where do you draw the line on who sits on Old Sparky and who sits on ice?
Samples sat on ice for 10 min and were then dried in a speed vac at 30°C for 10 min. Subsequently, 20 µl trypsin solution (12.5 ng of trypsin/µl digestion buffer [25 mM NH4HCO3, 10% acetonitrile, 5 mM CaCl2]) were added, and the samples were incubated at 4°C for 30 min followed by the addition of 60 µl of digestion buffer.
And if that wasn't disturbing enough, Temple described the director of "Baby Burlesks" punishing his very young actors for misbehaving by confining them to a windowless black box in which there was nothing to sit on but a block of ice.
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