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I am the one teaching him, "No Ziggy, sit, drop, come, good boy" and best of all I am the one who gets to give and receive unconditional love and cuddles all day long.
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Sitting drop crystallization experiments were performed at room temperature using a 1 1 ratio of DHQD (7.5 mg/mL) to reservoir.
Sitting drops composed of 200 nl protein mixed with 200 nl crystallization solution were equilibrated against a 50 µl reservoir at 277 K for 26 days prior to harvest.
Sitting drops were set up using a Mosquito crystallization robot (TTP Labtech, Royston UK).
Sitting drops were prepared by mixing 0.5 μl of fresh ctArx1 (15 mg/ml) with 0.5 μl of reservoir solution containing 0.2 M LiAcetate and 2.2 M (NH4 2SO4.
Sitting drops of 1 μl consisted of a 1 1 (volume:volume) mixture of protein and a well solution containing 0.15 M potassium thiocyanate, 0.1 M sodium cacodylate pH 6.5, 15% polyethylene glycol 6000 for the RPEL1 LatB actin complex, and 0.2 M sodium chloride, MES pH 6 and 20.5% polyethylene glycol 6000 for the RPEL2 LatB actin complex.
For the sitting drop method, the protein drop sits directly on a surface of the crystallisation plate above the crystallisation condition.
Sit back, drop your 12 points and surprise me with an answer.
Anyway, she and MK are here to sit and drop some knowledge on the final three.
Once he has returned, get him to sit and drop the keys.
When Tim was told to sit, he dropped down so fast it was hilarious.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com