Owing to the cross-talk between NF κB and ROS MAPK pathways, singly targeting either pathway may not be sufficient for inducing cancer cell killing.
Homoeologous genes were successfully targeted singly and together, demonstrating that CRISPR/Cas9 can both selectively, and generally, target members of gene families.
Accusing Shias of collaborating with Iraq's western occupiers, they have targeted them singly and in groups, at mosques, funerals, bus stations and anywhere frequented by the nascent police force largely manned by Shia recruits.The violence has clearly driven a wedge into Iraqi sensibilities.
Overall, 34 of 65 (52.3%) pairs of putative C. briggsae GLD-1 targets and 14 of 25 (56%) singly injected targets produced obvious germline RNAi knockdown phenotypes.
This nickase version of Cas9 can be targeted in pairs to increase specificity or singly to generate DNA nicks capable of promoting HDR but not NHEJ, although at a cost of reduced flexibility and efficiency (Cong et al. 2013; Hsu et al. 2013; Mali et al. 2013a).
Neurons can be visually identified and targeted for both extracellular and intracellular recordings made singly or in simultaneous pairs.
Increasing attention is drawn to the development of therapeutic strategies targeting CSCs, singly or in combination of traditional treatments targeting bulk tumor masses.
In addition, in the C181S mutation, which has single palmitoyl and is Golgi targeted like N-Ras, more rasosomes are created than in H-Ras-WT or the singly palmytoylated C184S.
Significantly, while the two MOs were singly or together co-injected with 5 ng/ μl zNoxa-rescued mRNA (rcRNA), which was not targeted by the zNoxa MO1 and MO2 through mismatching five nucleotides in zNoxa mRNA (Supplementary Material), the zNoxa protein expression was restored.
To singly biotinylate SSTR3 or Smo, IMCD3 cells stably expressing AP-SSTR3-GFP or AP-Smo-YFP were transfected with pDisplay/BirA-ER (BirA-ER is a biotin ligase targeted to the ER lumen) and RFP-Pericentrin PACT domain (a marker of the ciliary base), and maintained in DMEM/F12 supplemented with 0.2% FBS and 10 μM biotin (#BIO200; Avidity) for 24 hr.
