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At E14, doubly labelled cells were embedded between singly labelled GPT neurons born before and after E12.
Singly labelled cell aliquots along with cells stained with fluorochrome-labelled rat isotype control antibodies (BD Pharmingen, Franklin Lakes, New Jersey, USA) were used as compensation and negative staining controls, respectively.
Comparable ablated mice had many persistent, singly labelled BrdU cells in the ventricular zone until E14.
At E15 E16, a larger complement of doubly labelled cells born on E13 settled between singly labelled GPT neurons born before and after E13.
Of the 191 Golgi units, 139 (73%) were co-labelled with GM130 and GCC185, with the remaining 52 units (27%) singly labelled with either marker.
Adjacent sections singly labelled for each reporter showed that approx. 30% of the cells in the marginal zone in unablated triple-transgenic experimental mice were GPT neurons, with similar results for τ-eGFP and lacZ in control mice during this early period.
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A MUL-tree is a triple (T, M, ψ), where (i) T is an unrooted treea with leaf set L (T ) all of whose internal nodes have degree at least three, (ii) M is a set of labels, and (iii) ψ T : L (T ) → M is a surjective map that assigns each leaf of T a label from M. (Note that if ψ is a bijection, T is singly labeled; that is, singly-labeled trees are a special case of MUL-trees).
We performed 18 parallel labeling experiments, using all singly labeled glucose tracers for each strain, reconstructed and validated metabolic network models, measured biomass composition, and quantified precise metabolic fluxes for each organism.
NTFD works with nearly all stable isotopic-labeled compounds, including uniformly or singly labeled carbon, nitrogen, or sulfur tracers.
Oligomers are thus distinguished from monomers by the presence in both detection channels of simultaneous fluorescent signals, the intensity of which can be used to estimate the oligomerization order of the dual-labeled species (by comparison with the signal emitted by singly labeled monomeric species).
Singly labeled S90R1, singly labeled S117R1, and doubly labeled S90R1/S117R1 were prepared.
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CEO of Professional Science Editing for Scientists @ prosciediting.com