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A second pass might be unnecessary for single tilt series.
Fig. 11 Flow chart describing the automation process for a single tilt series reconstruction.
It appears the signal amplitude of a single tilt (N = 1) reconstruction is higher compared to a 16-tilt reconstruction.
Tilt series were collected along a single tilt axis (Fig. 4B) with attentions paid to avoid the electron irradiation damage.
It is clear that having detected a maximum number of markers during the single tilt series alignment is important to establish the geometrical correspondence between tilt series.
This appendix describes in detail our procedure steps: (i) the marker detection, (ii) the single tilt alignment, (iii) the multiple tilt alignment.
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A window-type environmental cell (EC) that incorporates a uniaxial straining apparatus is built into a side-entry-type single-tilt specimen holder.
However, the list of Kindle Singles tilts heavily toward the dark and depressing.
A modified single-tilt TEM/STEM specimen holder containing a slot fitting the liquid enclosure (Figure 4) was used to position the micro-environmental chamber in the STEM.
To obtain an optimal coverage of the complexes during a single-tilt tomographic image acquisition, the long axis of the complexes had to run either parallel to the tilt axis or parallel to the beam axis of the microscope.
Even a single engine tilt adjustment at the beginning of deflection operations yields more deflection than a fixed preset tilt.
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