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No viral RNA shedding was recorded for two birds (M1 and M2) whereas low viral RNA excretion (high ct-value) was detected in a single swab for three birds (M4, M6 and M5).
Overall, this model predicted that the probability of detecting AIV for a single swab collected within 8 dpi, was 33.0% (95% CI = 30.1, 47.3) for a cloacal swab, 44.2% (95% CI = 35.6, 53.1) for an oropharyngeal swab, and 52.1% (95% CI = 45.76, 58.34) for a fecal swab equivalent.
For the nose specimen, a single swab is used to sample each nostril.
If growth was documented only in a single swab, colonization time was defined arbitrarily as three days.
Each yeast sample was collected by scraping the dorsum of the tongue with a sterile cotton swab (COPAN, Amies Charcoal single swab, CE 0124, Italy).
Mouth samples were obtained by firmly rubbing the dors of the tongue, mucosal part of the cheeks and the hard palate, using a single swab.
Similar(45)
Single swabs from virus inoculated birds were included as a control.
The experiment was conducted so that for each time point there were 15 individual pools of 5 and 11 swabs and 10 single swabs.
The titer of virus based on rRT-PCR was compared between each swab pool, and on 2 DPI and 4 DPI, single swabs had significantly lower titers of virus as compared to 5 or 11 swab pools.
An identical experiment was conducted to evaluate swab pooling with NDV, The experiment was conducted so that there were 19 pools of 5 and 11 swabs and 10 single swabs.
Two identical replicates of the experiments were conducted for a total of 20 single swabs, 38 pools of 5 and 38 pools of 11 for each day for both OP and CL.
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