Sentence examples for single stringency from inspiring English sources

The development of DNA microarray assays is hampered by two important aspects: processing of the microarrays is done under a single stringency condition, and characteristics such as melting temperature are difficult to predict for immobilized probes.

Additionally, C2 re-aligned region must coincide with the C1 anchor region with single nucleotide stringency.

We evaluated the use of low-stringency single specific primer PCR (LSSP-PCR) for genetically typing Leptospira directly from urine samples of cattle with clinical suspicion of leptospirosis.

Using the sensitive DNA profiling technique, LSSP-PCR (Low-Stringency single Specific Primer PCR), we previously showedthat hearts of BALB/c mice doubly infected with JG and Col1.7G2 T. cruzi populations were mainly colonized by JG strain, while Col1.7G2 was preponderant in the rectum and all other analyzed tissues [2].

In localization microscopy, the stringency of single molecule detection always influences the structural resolution.

The screening was successfully re-performed with a single round of high stringency.

Alternatively, positive clones could be located at the ends of different contigs that do not have enough overlap to be assembled into a single contig under the stringency used for the analysis.

Because longer scaffolds are more likely contain sequences belonging to multiple BACs (i.e. lower proportion of contigs originating from a single BAC) and scaffolding stringency affect scaffold length distribution, we normalized the scaffold reliability by dividing on scaffold length (i.e. proportion of contigs in a scaffold with a BLASTN hit to BAC/scaffold length).

The latter was a single, one-step reaction under high stringency conditions, using simultaneously 2 pairs of primers (1 pair for C. sinensis product size 612 bp, and 1 pair for O. viverrini product size 1357 bp).

Briefly, kDNA amplicons were subjected to electrophoresis in 1.5% agarose gel (1.0% agarose, 0.5% agarose low melting point), punctured from the gel, diluted 10 times in double distilled water, and submitted to a second step of low stringency amplification, using a single primer (S35G: 5'-ATGTACGGGGAGATGCATGA-3').

Disregarding the latter contig and the four singletons, all control fingerprints from the three libraries assembled into a single contig at very high assembly stringency.