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To estimate the reduction of normal-looking neurons, we counted the number of positive cells for the different LSG in a single section for each sample using Image Pro Plus 7.0 software (Media Cybernetics, Bethesta, MD, USA).
Three nonadjacent sections from each animal were analysed for PGP 9.5; a further three separate sections for GAP-43 (for paw pad and back skin); and a single section for CGRP, SP, and β-tubulin (for paw pad skin only).
A subset of sections was first immunostained for OC and visualization with Alexa Fluor 568 and then counterstained with thioflavin S. Immunostaining by the OC antibody was captured from a single section for each AD, DS and control case using a 20× objective, a Sony high-resolution CCD video camera (XC-77) and the built-in video capture capabilities of a Macintosh 8100/80AV.
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Methods: MicroCT was carried out on cones using a General Electric phoenix v|tome|x s 240D, and resulting projections were processed with visualization software to produce image stacks of serial single sections for two-dimensional (2D) visualization, 3D segmented reconstructions with targeted structures in color, and computer animations.
TESS will monitor thousands of stars simultaneously for such "transits," watching a single section of sky for a month straight before moving on to another.
The insets show a separate channel presentation of a representative optical single section of the TB, for details refer to the image stack in Figure 9 source data 6 that was used for this projection.
A single section of aortic root was used for each of the separate staining protocols per animal.
For single section movies, time-lapse imaging datasets were manually assembled using ZEN software, and then the brightness and contrast of movies were adjusted using Fiji.
The optical single sections used for the projection are presented in Figure 10 source data 1.
The optical single sections used for this projection are deposited in Figure 8 source data 1.
Movie 2 shows the progress of the optimization on three serial sections imaged as 4 × 4 tile mosaics demonstrating that the procedure for multiple section is essentially the same as for a single section.
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