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While replication-competent adenoviruses that directly encode therapeutic transgenes have been investigated [ 4], a two-virus system such as that described have may be particularly useful in cases where total transgene size is too large to engineer into a single replicating virus.
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This program uses a Bayesian MCMC search strategy of a single, replicated, two million step chain.
Single replicate analyses have generated consistent results in previous studies [ 30, 31, 74], but confirmation of some of the results obtained from digital expression analysis was performed with RT-PCR on four new experimental replicates (see below).
From this set of materials, 293 inbreds and their B73-hybrids were grown in adjacent one-row plots in a single replicate, along with replicated B73 plots, in Homestead, FL during the winter of 2002.
Each institution was treated as a single replicate, regardless of the number of students belonging to it.
Furthermore, there was only a single replicate of each selected treatment combination, so that the experiment consisted of 25 plots, each assigned a different treatment combination.
Due to the small size of the samples compared to the beam size, only a single replicate could be performed on each fragment, and the contribution of the embedding resin cannot be excluded.
Sterility of adult female A. ferus from a single replicate treated at 50 to 200 Gy was 99.9% at all treatment levels after correcting for control mortality of 11.7% (Fig. 1).
It was calculated for each single replicate.
One single replicate consists of one 96-well plate.
While multiple hybridization replicates provided ∼97% accuracy, even a single replicate provided ∼95% accuracy.
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