Sentence examples for single protocol was used from inspiring English sources

A single protocol was used, derived from the EUROCARE high-resolution protocols.

Therefore, a single injection protocol was used for further experiments.

A single PCR protocol was used to test all primers on every sample using a Biometra "TProfessional" thermal cycler.

A single knockdown transfection protocol was used.

For all loci a single touchdown PCR protocol was used, decreasing the annealing temperature from 68°C to 48°C [ 67].

The 5′ end of one of each primer pair was fluorescently labeled (Table 1) and the following single-step PCR protocol was used: in 20-μL reaction volumes containing approximately 20 ng DNA template, 0.5 U Platinum Taq (Life Technologies, Carlsbad, California, USA), 2 μL Platinum Taq PCR buffer, 0.1 mM dNTPs, 2 mM MgCl2, 0.2 μM of each primer, and sterile water to 20 μL.

The same transformation protocol was used for single transformations as well as for transformations in the 96-well format.

A similar protocol was used by Siegler et al. to achieve single-KIR+ NK cells using CliniMACS [ 71].

A standard immunohistochemical protocol was used [21].

A 'double hit' oligonucleotide transfection protocol using oligofectamine transfection reagent (Invitrogen) was employed for siRNA transfection, as described previously (12), except prior to luciferase assays, where a 'single hit' protocol was used.

Standard protocol was used.