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It's a different story when a town prohibits the stolpersteine, as has happened in Munich, the only big German city without a single plaque.
Usually, progeny viruses generated by co-infection of two distinguishable parental strains are first cloned by selection of a single plaque and then characterised by PCR.
Instead, they were honored by a single plaque, referring to them only as "Mexican nationals".
A single plaque from each plate was selected and plated with the host to establish a genetically homogenous virus population.
If the patient had only one plaque, the single plaque was large in size (greater than 1% of total body area).
Four days following the plaque assay, a single plaque from each virus was isolated and used for a second round of plaque purification in MDCK cells.
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To recover the 402 bp gene 20 deletion mutant, ten mixed plaques were picked and re-plated and single plaques tested by PCR; at least one pure mutant was identified in seven of these, although at greatly varying frequencies (1/15, 2/15, 3/16, 10/15, 2/17, 2/24, 2/15; 19.8% average).
All phages were purified by three consecutive single plaque-assays.
In the case of ME49, a single plaque-purified clone (i.e. ME49 B7.21-E1) that was FUDRr was further passaged in a cat (cross TX332) to derive the parental clone referred to as ME49 FUDRr.
We isolated a single plaque-purified copy of clone C1 that had evolved on ERA at large population sizes; sequencing confirmed that this clone contained the F176L mutation presumably beneficial for ERA infection.
Single plaques were subcultured three times for phage purification.
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