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For timelapse imaging experiments, a single plane of focus was used to capture movies (1 frame/min) and this was done to minimize photobleaching and toxicity.
A single plane of focus in the filled neuron showing postsynaptic α-btx label, peak Fluo-4 calcium signal and merged α-btx and Fluo-4 signal.
Images of cilia were captured from randomly chosen high-power fields (40× objective), when the full extent of cilia could be visualized in a single plane of focus.
Bythotrephes were oriented on their side such that the length of the body and tail spine was along a single plane of focus and the eye, thorax and abdomen regions of the body were visible (Fig. 1).
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The large separation distances between adjacent lattice sites enable optical addressing of individual atoms using a shallow depth of focus lens such that only a single plane of atoms is in focus at a time.
On occasion (as indicated in the text) a single plane of the Z-stack was used.
Single plane of confocal z-stack picture was opened in ImageJ.
Each neuronal cell body was imaged at its widest diameter in the plane of focus.
Also it was not possible to capture all the signals in one plane of focus.
One important parameter is the selection of an appropriate plane of focus.
Videos were rejected if the plane of focus altered significantly during imaging acquisition.
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