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Danev, R. & Baumeister, W. Cryo-EM single particle analysis with the Volta phase plate.
Moriya, T. et al. High-resolution single particle analysis from electron cryo-microscopy images using SPHIRE.
Danev, R., Tegunov, D. & Baumeister, W. Using the Volta phase plate with defocus for cryo-EM single particle analysis.
Our study worked on two aspects, the improvement of protein quality for structural analysis and performing single particle analysis cryo-EM.
Here, we use cryo-electron microscopy (cryo-EM) and single particle analysis to gain structural insight into the full-length dimeric structures of LRRK2 and LRRK1.
Thus far, protein complexes that have been successfully reconstructed to high resolution by single particle analysis (SPA) have molecular weights of ∼100 kDa or larger2.
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Extended Data Fig. 2 Cryo-EM images and single-particle analysis of the Rho Gi Fab complex.
In summary, single-particle analysis of the fluorescent HIV viruses provides a valuable tool for assessing the IN content and oligomeric/conformational state of viral complexes during infection.
Zivanov, J., Nakane, T. & Scheres, S. H. W. A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis.
Here, the structure of human TPPII (HsTPPII) has been determined to subnanometer resolution by cryoelectron microscopy and single-particle analysis.
Two recent papers describe near-complete atomic structures of these complexes based on cryoEM single-particle analysis.
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