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On the other hand, two single mutations (S346A and T347A) did not affect the binding outcomes.
Take for instance, progeria or achondroplasia, where single mutations bring about multi-compartment changes.
We have also made a double mutant, combining two of the most beneficial single mutations.
We have developed a structure-based method to rationally predict single mutations at protein protein interfaces that enhance binding affinities.
Both multiple mutants display large modifications of stability and activity arising from synergic effects in comparison with single mutations.
The restriction analysis of nrDNA with the restriction enzymes allowed to detect the presence of two single mutations (SNPs) among the sequences of two species.
Although most of the single mutations had no effect, we cannot exclude the possibility that different phosphorylation sites may combine together to play a role in autophagy.
First, single mutations were made on all 6 lysines.
All mutations, including single mutations, were included in this calculation.
Moreover, interactions between specific single drugs and single mutations or pairs of drugs and single mutations are represented by additional covariates.
In the double mutants and a triple mutant, the single mutations were incorporated in various combinations.
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