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To generate fls2 double-1 double mutant, single mutant fls2 was crossed with desi3a-1 single mutant to generate fls2 double-1 double mutant.
This connection has since been identified as a single mutant gene.
(A) Circulating IgE levels were determined in the double and single mutant animals.
Low levels of YUC4 expression were also found in the crc single mutant (Fig. 2c).
The findings hint that a single mutant copy of this gene makes people more susceptible.
This is manifested as a loss of sensitivity to CO2 in the single mutant proteins.
Using sequence analysis and site-directed mutagenesis, an additional single mutant was then produced, with 500-fold improved activity.
SUMOylation of wild-type G9a and single mutant proteins was assessed by the Global Protein SUMOylation assay kit (Abcam, ab115131).
The dnmt3ab double mutant lost 95% of CHH methylation, which is comparable to the CHH loss in dnmt3b single mutant.
Several dog breeds have short legs, for example, but only a single mutant gene is responsible for the change.
Two mutant enzymes were predicted: the single mutant S262A and the double mutant F218Y-S262A.
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