The ability to perform up to a million parallel hybridisations on a single microarray provides researchers with a very efficient platform for characterisation of genetic processes in target organisms.
For expression analysis, publicly available data for single microarray platform – 51 K Affymetrix gene chip was used since it covers most of the rice genes and the maximum microarray data in the public repositories has been generated using these chips.
This allowed analysis of multiple samples on a single microarray.
TRANSMODIS is an extension to MODEM, which was developed for analyzing a single microarray experiment.
Each mRNA sample extracted from an individual sample was run on a single microarray.
The usual approach is to analyse a single microarray dataset in isolation.
Biological replicates from Florida were used to interrogate a single microarray slide each.
In this configuration, a single microarray supported 200 different experimental conditions with respect to Ppy deposition and Ag concentrations.
Developments over recent years have allowed researchers to extend the interrogation of expression to all known genes of a certain organism using a single microarray.
The fluorescent signal of each hexamer sequence on a single microarray chip, quantitatively reflects the expansion of a certain T-cell clone.
However, the size of the TCRβ repertoire at the DNA level is too large for full TCR repertoire analysis at single-clone resolution on a single microarray.
