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Nevertheless, the indirect method also has limitations, being applicable only to the single method used in the population sample considered in that particular study.
There was no single method used for anything.
Eighteen studies used patient satisfaction as an outcome, with no single method used consistently.
Contact with authors was the single method used in this instance.
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Real-time quantitative RT (qRT PCR was performed with a single method using the LightCycler FastStart DNA Master SYBR Green I kit (Roche, Mannheim, Germany).
Real-time quantitative RT-PCR was performed with a single method using a LightCycler FastStart DNA Master SYBR Green 1 Kit (Roche Diagnostics GmbH, Mannheim, Germany).
Real-time quantitative RT PCR was performed with a single method using a LightCycler FastStart DNA Master SYBR Green I kit (Roche Diagnostics GmbH, Mannheim, Germany).
Real-time quantitative reverse transcriptase polymerase chain reaction (qRT PCR) was performed with a single method using a LightCycler FastStart DNA Master SYBR Green 1 Kit (Roche Diagnostics GmbH, Mannheim, Germany), according to the procedure provided by the manufacturer.
Quantitative reverse transcriptase polymerase chain reaction was performed to validate mRNA expression with a single method using a LightCycler FastStart DNA Master SYBR Green I kit (Roche Diagnostics GmbH), according to the procedure provided by the manufacturer.
Comparison study - single-gene approach: The single-gene method used was the moderated t-statistic, implemented via the lmFit and eBayes functions from the limma package [ 30] in R [ 53].
Young's modulus was estimated by the single beam method using finite element modeling.
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