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The conventional device has a single isolation peak in the X-band.
Conclusions: Our preliminary analysis suggests a possible beneficial effect of single isolation rooms in reducing NI rate in the PICU.
Quadruplicate measurements were made on a single isolation of RNA from each cell line analyzed.
The dominant pair were kept overnight in single isolation nets within their compartments before being released.
Thus, a single SIM (i.e., a historical model with a single isolation time) may not be compatible with both data sets.
Cells were picked using a sterile extended pasteur pipette from a dish, washed in fresh autoclaved seawater to check for single isolation under a microscope, photographed and picked using a separate autoclaved, pipette.
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This represented single isolations from 12 patients and 23 repeat isolations (2 to 5 isolates from 7 patients each) from clinically significant findings or those isolated from weekly surveillance cultures.
For single-spore isolation, single conidia were collected and spread on Bacto agar medium with a fine glass needle.
Initial steps for single cell isolation were identical to those used for organoid isolation as described above.
The isolated phages were then diluted and purified twice by single plaque isolation (13 ).
Fig. 1 The current methods for single cell isolation.
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