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SERS spectra were recorded with the same Bruker Senterra Raman instrument, using a 20x long working distance microscope objective and excitation at 488 nm, with a single integration of 30 s. SERS analyses were executed by focusing onto the microaggregates which were formed inside the dye-colloid droplet a few seconds after the deposition of the Ag nanoparticles and KNO3.
Single integration of the deletion construct was verified by Southern blot (data not shown).
Correct targeting events (Fig1) and single integration of the targeting vector (Figure S1) were confirmed by Southern blotting.
Southern blot analysis of the 4 lines confirmed that a single integration of the transposable element had occurred in each line (Figure 1b).
DNA blot hybridization results presented in Figure 3 indicated a total of 13 lines out of the 22 evaluated appeared to contain a single integration of the transgene.
Each 35S DR2 line carries a different, single integration of the CaMV35S: HaDREB2 transgene in heterozygosis.
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Braun, V., Hundsberger, T., Leukel, P., Sauerborn, M. & Von EiC.el-Streiber, C. Definition of the single integration site of the pathogenicity locus in Clostridium difficile.
Confirmation of the single integration event of the pC-P54::MeCu/ZnSOD-35S::MeAPX2 T-DNA in these transgenic lines were carried out by the Southern blotting technique using XbaI-digested cassava genomic DNA, which were extracted from leaves of in vitro plants and hybridized with DIG-labeled HPT probe.
Indeed, they match an evolutionary scenario of endosymbiogenesis during which, from a single integration event of symbiotic virus genome, viral genes were lost and/or translocated from the provirus to other chromosomal regions (Fig. 5).
We confirmed a single integration site of the Hermes retrotransposon by Southern blotting (Fig EV1B).
Initial experiments were performed with DS10 A9/DR2 lines that contained different, single, integration sites of the DS10 HaDREB2 transgene in heterozygosis.
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